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Botulinum toxin is a type of protein produced by Clostridium botulinum which acts on the presynaptic nerve endings and blocks acetylcholine secretion. The resulting muscle paresis effect is used clinically to treat a number of diseases related to overactive muscle activity.
Botulinum toxin A is sold in a freeze-dried state and must be diluted prior to clinical use. Since potency of the toxin gradually decreases after dilution, manufacturers recommend that it should be used within 4 hours after dilution with 0.9% saline solution.
Potency after dilution may be affected not only by time, but also by the type of diluent. Mclellan et al. used a mouse lethality assay to show that botulinum toxin A diluted with saline lost twice as much potency than did toxin diluted with buffer solution containing 0.2% gelatin. Schantz and Johnson  reported a loss of toxin stability resulting from dilution of botulinum toxin A, and that diluents containing gelatin or albumin could prevent such loss.
Botulinums/Diluents Products at Mega Shop Includes the following